Parenteral Phase
Summary of morphology and development of L1
Summary of nurse cell formation. Letters in time course at top correspond to images below.
Bolus of newborn larvae injected into mouse muscle. 10 min post-injection. H&E
Newborn larva entering muscle cell 10 min post-injection. H&E. Note stretched sarcolemma, indicating worm has not yet entered cell cytoplasm.
Newborn larva entering muscle cell 10 min post-injection. H&E
Newborn larva entering muscle cell. Thick section. Note damage to sarcolemma.
Newborn larva inside muscle cell. 1 hr post-injection. TEM
L1 inside developing nurse cell. 4 days post-injection. Note vacuole around worm. Thick epon section.
L1 inside developing nurse cell. 4 days post-injection. Note lack of integration of host cell cytoplasm with larval cuticular surface. TEM
L1 inside muscle cells. 5 days post-injection. H&E
L1 in developing nurse cells. 6 days post-injection. H&E
L1 in developing nurse cell. 7 days post-injection. H&E
L1 in developing nurse cell. 8 days post-injection. Note worm has yet to coil up. Thick epon section.
L1 in developing nurse cell. 10 days post-injection. H&E
L1 in developing nurse cell. 10 days post-injection. Thick epon section.
L1 in developing nurse cell. 14 days post-injection. Note coiled worm. H&E
L1 in developing nurse cell. 15 days post-injection. Parasite now infectious for a new host. Thick epon section.
Mature nurse cell-parasite complex. Note intense inflammatory response. H&E
Mature nurse cell-parasite complex. Thick epon section.
Isolated nurse cell-parasite complex. Photomicrograph by Eric Gravé. Winner of 1976 Nikon Small World Contest.
EMs of Selected Stichocytes of Mature L1
L1 0 hr SEM
L1 stichosome
Summary of stichocyte cell types
L1 esophagus and duct to stichocyte. Note cuticular lining of gut tract and duct. High magnification TEM.
L1 esophagus at the level of nerve ring. TEM
L1 midgut. TEM
L1 alpha and beta stichocytes. Longitudinal section. TEM
L1 alpha stichocyte TEM
L1 alpha stichocyte granules, higher magnification. Note prominent Golgi. TEM
L1 alpha stichocyte. Note canalicular tree and portion of duct. TEM
L1 alpha 2 granule. TEM
L1 alpha 2 and beta 2 granules. TEM
L1 beta stichocyte. Note duct. TEM
L1 gut tract and duct of beta stichocyte. TEM
L1 beta 1 stichocyte high magnification. TEM
L1 beta stichocyte beta 1 granule. TEM
L1 beta 2 granule. TEM
L1 8 hr post oral infection, beta 1 stichocyte. Note duct. TEM
L1 8 hr post oral infection, beta 1 stichocyte, higher magnfication. Note duct. TEM